Cataloguenumber
PKA-234
Synonyms
Introduction
Description
FLT1D1-3HumanRecombinantproducedinbaculovirusismonomeric,glycosylated,polypeptidecontaining327aminoacidsandhavingamolecularmassof45kDa.
Thesolublereceptorproteincontainsonlythefirst3extracellulardomains,whichcontainalltheinformationnecessaryforbindingofVEGF.
TheFLT1ispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
FLT1D1-3waslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontaining1xPBS.
Solubility
StABIlity
LyophilizedFLT-1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFLT1shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BiologicalActivity
TheactivityofFLT1D1-3wasdeterminedbyitsabilitytoinhibittheVEGF-165-inducedproliferationofHUVEcells.
Aminoacidsequence
SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGKRSY.