Cataloguenumber
ENZ-138
Synonyms
Ubiquitin-foldmodifier-conjugatingenzyme1,Ufm1-conjugatingenzyme1,UFC1,CGI-126,HSPC155.
Introduction
UFC1isamemberoftheubiquitin-conjugatingenzymefamily.UFC1isanE2-likeconjugatingenzymeforubiquitin-foldmodifier-1.UFM1isactivatedbyUBA5(anovelE1-likeenzyme)byformingahigh-energythioesterbond.ActivatedUFM1issubsequentlytransferredtoitscognateE2-likeenzyme,UFC1,inasimilarthioesterlinkage.
Description
UFC1producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining187aminoacids(1-167a.a.)andhavingamolecularmassof21.6kDa.
UFC1isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
UFC1proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glycerol,0.1MNaCland1mMDTT.
StABIlity
UFC1HumanRecombinantalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKCWYIHDLLKYEFDIEFDIPITYPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEIPDLIQKGVIQHKEKCNQ.