Cataloguenumber
ENZ-396
Synonyms
Nedd-8,FLJ43224,MGC104393,MGC125896,MGC125897,NEDD8,Ubiquitin-likeproteinNedd8,Neddylin,Neuralprecursorcellexpresseddevelopmentallydown-regulatedprotein8.
Introduction
NEDD8ispartoftheubiquitinfamily.HumanNEDD8shares60%aminoacidsequencehomologytoubiquitin.TheNEDD8systemisessentialfortheregulationofproteindegradationpathwaysinvolvedincellcycleprogression,morphogenesisandtum
Origenesis.NEDD8isinvolvedincellcyclecontrolandembryogenesis.CovalentattachmenttoitssubstratesrequiresprioractivationbytheE-1complexUbE1c-appbp1andlinkagetotheE-2enzymeUbE2m.AttachmentofNEDD8tocullinsactivatestheirassociatedE-3ubiquitinligaseactivity,andthuspromotespolyubiquitinationandproteasomaldegradationofcyclinsandotherregulatoryproteins.
Description
NEDD8HumanRecombinantfusedwith37aminoacidHistagatN-terminusproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining113aminoacids(1-76a.a.)andhavingamolecularmassof12.8kDa.
TheNEDD8ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheNEDD8solutioncontains20mMTrispH8.0,50mMNaCl,0.5mMDTT&10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MHHHHHHMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEA
VNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGRGSHMAAAEAANCIMEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLHARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYG
FDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.