
Cataloguenumber
ENZ-1038
Synonyms
Tyrosyl-DNAphosphodiesterase1,Tyr-DNAphosphodiesterase1,TDP1,FLJ11090,MGC104252.
Introduction
TDP1belongstothephospholipaseDfamilyandcontainstwoPLDphosphodiesterasedomains.TDP1isinvolvedinrepairingstalledtopoisomeraseI-DNAcomplexesbycatalyzingthehydrolysisofthephosphodiesterbondbetweenthetyrosineresidueoftopoisomeraseIandthe3-primephosphateofDNA.TDP1mayalsoremoveglycolatefromsingle-strandedDNAcontaining3-primephosphoglycolate,suggestingaroleinrepairoffree-rADIcalmediatedDNAdouble-strandbreaks.MutationsintheTDP1genearelinkedtothediseasespinocerebellarataxiawithaxonalneuropathy(SCAN1).
Description
TDP1HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining617aminoacids(1-608)andhavingamolecularmassof69.5kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).TDP1isfusedto9aminoacidHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TDP1proteinsolution(0.25mg/ml)containingPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPMSQEGDYGRWTISSSDESEEEKPKPDKPSTSSLLCARQGAANEPRYTCSEAQKAAHKRKISPVKFSNTDSVLPPKRQKSGSQEDLGWCLSSSDDELQPEMPQKQAEKVVIKKEKDISAPNDGTAQRTENHGAPACHRLKEEEDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSAQFNYCFDVDWLVKQYPPEFRKKPILLVHGDKREAKAHLHAQAKPYENISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIHADWHQKTQGIWLSPLYPRIADGTHKSGESPTHFKADLISYLMAYNAPSLKEWIDVIHKHDLSETNVYLIGSTPGRFQGSQKDNWGHFRLKKLLKDHASSMPNAESWPVVGQFSSVGSLGADESKWLCSEFKESMLTLGKESKTPGKSSVPLYLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQNWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKIAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFGLDSFKVKQKFFAGSQEPMATFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPSHHHHHH.