Cataloguenumber
PKA-094
Synonyms
Receptortyrosine-proteinkinaseerbB-2,EC2.7.10.1,p185erbB2,C-erbB-2,NEUproto-oncogene,Tyrosinekinase-typecellsurfacereceptorHER2,MLN19,CD340antigen,NEU,NGL,HER2,TKR1,HER-2,c-erbB2,HER-2/neu.
Introduction
HER-2/neu(erbB-2)encodesan185-kDaorphanreceptortyrosinekinasethatisconstitutivelyactiveasadimeranddisplayspotentoncogenicactivitywhenoverexpressed.Herstatin,astheproductofalternativeHER-2transcript,retainsintron8.
TheherstatinmRNAisexpressedinnormalhumanfetalkidneyandliver,butisatreducedlevelsrelativetop185HER-2mRNAincarcinomacellsthatcontainanamplifiedHER-2gene.Herstatinappearstobeaninhibitorofp185HER-2,becauseitdisruptsdimers,reducestyrosinephosphorylationofp185,andinhibitstheanchorage-independentgrowthoftransformedcellsthatoverexpressHER-2.
Description
ErbB2HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining638aminoacids(23-652)andhavingamolecularmassof70.4kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).ErbB2isfusedto8aminoacidIgGHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
ErbB2proteinsolution(0.25mg/ml)containingPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTLEHHHHHH.
