Cataloguenumber
ENZ-1057
Synonyms
UridineMonophosphateSynthetase,UMPSynthase,OrotatePhosphoribosylTransferaseAndOrotidine-5-Decarboxylase,Orotidine5-PhosphateDecarboxylase,OrotatePhosphoribosyltransferase,Uridine5-MonophosphateSynthase,OMPdecase,OPRTase,OPRT,Uridine5"-monophosphatesynthase,UMPsynthase,Orotatephosphoribosyltransferase,Orotidine5"-phosphatedecarboxylase,ODC.
Introduction
Uridine5"-monophosphatesynthase(UMPS),isabifunctionalenzymethatcatalyzestheultimatetwostepsofthedenovopyrimidinebiosyntheticpathway.UMPSineukaryoteslinkstheorotatephosphoribosyltransferaseandtheorotidine-5’-monophosphate(OMP)decarboxylaseactivitiesintoasingleprotein.Theharmonyofthese2enzymesisassumedtobestABIlizedthecatalyticcentersasaresultofthelowmolarconcentrationoftheproteininmammaliancells.MutationsinUMPS arethereasonofinheritedoroticaciduriadisease.
Description
UMPSHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining486aminoacids(1-480a.a.)andhavingamolecularmassof53kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).UMPSisexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
UMPSproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4).
Stability
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIVSRPRLLSQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKETKDYGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAIVLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSIKEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWEAYLSRLGVHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.