Cataloguenumber
ENZ-140
Synonyms
Uroporphyrinogen-IIIsynthase,UROIIIS,UROS,Hydroxymethylbilanehydrolyase[cyclizing],Uroporphyrinogen-IIIcosynthase.
Introduction
UroporphyrinogenIIIsynthase(UROS)isanenzymeinvolvedinthe4thstepofporphyrinmetabolismandintheconversionofhydroxymethylbilaneintouroporphyrinogenIII.DefectsintheUROSproteincancausemolecularlesionswhichleadtotheautosomalrecessiveGuntherdisease,otherwiseknownascongenitalerythropoieticporphyria(CEP).
Description
UROSproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining285aminoacids(1-265a.a.)andhavingamolecularmassof30.7kDa.
UROSisfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
UROSproteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),10%glyceroland0.1MNaCl.
StABIlity
UROSHumanRecombinantalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQPHGCC.

