Cataloguenumber
ENZ-163
Synonyms
Dihydropteridinereductase,HDHPR,Quinoiddihydropteridinereductase,QDPR,DHPR,PKU2,SDR33C1.
Introduction
QDPRbelongstotheshort-chaindehydrogenases/reductase(SDR)familyofenzymes.Operatingasahomodimer,QDPRhasanimperativeroleintherecyclingoftetrahydrobiopterin(BH4),avitalcofactorforthehydroxylationofthearomaticaminoacids(tryptophan,tyrosineandphenylalanine).Moreprecisely,QDPRcatalyzestheregenerationofBH4fromquinonoiddihydrobiopterin(qBH2),theproductgeneratedfromthehydroxylationreactions.MutationsintheQDPRgenemayleadtophenylketonuriaII.
Description
QDPRHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining267aminoacids(1-244a.a.)andhavingamolecularmassof28.2kDa.
QDPRisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMAAAAAAGEARRVLVYGGRGALGSRCVQAFRARNWWVASVDVVENEEASASIIVKMTDSFTEQADQVTAEVGKLLGEEKVDAILCVAGGWAGGNAKSKSLFKNCDLMWKQSIWTSTISSHLATKHLKEGGLLTLAGAKAALDGTPGMIGYGMAKGAVHQLCQSLAGKNSGMPPGAAAIAVLPVTLDTPMNRKSMPEADFSSWTPLEFLVETFHDWITGKNRPSSGSLIQVVTTEGRTELTPAYF.