Cataloguenumber
ENZ-131
Synonyms
Phosphoadenosinephosphosulfatereductase,3"-phosphoadenylylsulfatereductase,PAPSreductase,thioredoxindependent,PAPSsulfotransferase,PAdoPSreductase,cysH,b2762,JW2732.
Introduction
CysH(Phosphoadenosinephosphosulfatereductase)isamemberofthePAPSreductasefamily,specificallythoseactingonasulfurgroupofdonorswithadisulfideasacceptor.The3substratesoftheCysHenzymeareadenosine3",5"-bisphosphate,sulfite,andthioredoxindisulfide,whereasitstwoproductsare3"-phosphoadenylylsulfateandthioredoxin.
Description
CYSHproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining264aminoacids(1-244a.a.)andhavingamolecularmassof30.1kDa.
CYSHisfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
StABIlity
CYSHE.ColiRecombinantalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNLPGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLKVYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQSGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTTRKWEPGMAEEETRFFGLKRECGLHEG.

