
Cataloguenumber
PKA-007
Synonyms
Tyrosine-proteinkinaseSYK,Spleentyrosinekinase,p72-Syk,SYK.
Introduction
SpleenTyrosineKinase(SYK)belongstothefamilyofnon-receptortypeTyrproteinkinases.TheSYKproteinisextensivelyexpressedinhematopoieticcellsandisinvolvedincouplingactivatedimmunoreceptorstodownstreamsignalingeventswhichmediatediversecellularresponses,includingproliferation,differentiation,andphagocytosis.SYKisconsideredtobeamodulatorofepithelialcellgrowthandalikelytumorsuppressorinhumanbreastcarcinomas.SYKisapositiveeffectorofBCR-stimulatedresponses.SYKconnectstheB-cellantigenreceptor(BCR)totheutilizationofcalciumioneitherthroughaphosphoinositide3-kinase-dependentpathway,whennotphosphorylatedontyrosinesofthelinkerregion,orthroughaphospholipaseC-gamma-dependentpathway,whenphosphorylatedonTyr-348andTyr-352.ThereforethedifferentialphosphorylationofSykcandefinethepathwaybywhichBCRiscoupledtotheregulationofintracellularcalciumion(Bysimilarity).SYKalsophosphorylatesUSP25andregulatesitsintracellularlevels.
Description
SYKHumanRecombinantfulllengthprotein(1-635aa)producedinHEK293cellswithanN-terminalHis-Flagtag,havingamolecularweightof74.25kDa.
HumanSYKispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
SYKproteinissuppliedin50mMTrispH7.5,300mMNaCland10%Glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Pleaseavoidfreezethawcycles.
Purity
Aminoacidsequence
MHHHHHHDYKDDDDKKLMASSGMADSANHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGKISREESEQIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRADENYYKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPAGCPREMYDLMNLCWTYDVENRPGFAAVELRLRNYYYDVVN.