Cataloguenumber
PKA-102
Synonyms
Mast/stemcellgrowthfactorreceptorKit,SCFR,Piebaldtraitprotein,PBT,Proto-oncogenec-Kit,Tyrosine-proteinkinaseKit,p145c-kit,v-kitHardy-Zuckerman4felinesarcomaviraloncogenehomolog,CD117.
Introduction
KIT,alsoknownasKITProto-OncogeneReceptorTyrosine,isacytokinereceptorwhichisexpressednotonlyinhematopoieticstemcellsbutlikewiseinothercelltypes.KITbindstoreceptortyrosinekinasetypeIII,whichisastemcellfactor,alsonameda"rigidfactor"or"c-kitligand".Oncethisreceptorbindstostemcellfactor(SCF),itformsadimerwhichactivatesintrinsictyrosinekinaseactivity,whichsequentiallyphosphorylates&activatessignalingmoleculeswhichbreedsignalsincells.Thisreceptorprotectsvascularsmoothmusclecellsfromapoptosisinadditiontorestoringcardiacfunctionaftermyocardialinfarction.
Description
KITproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining507aminoacids(26-524a.a.)andhavingamolecularmassof57.1kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).
KITisexpressedwithan8aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculovirus.
PhysicalAppearance
Formulation
KITproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
QPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGT
EGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQSSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKGNNKEQIHPHTLFTPLEHHHHHH.