Cataloguenumber
PKA-264
Synonyms
Introduction
(ATP+D-galactose=ADP+alpha-D-galactose1-phosphate).GALK1deficinecyleadgalactosemiaIIwhichisanautosomalrecessivedeficiencyknownbycongenitalcataractsduringinfancyandpresenilecataractsintheadultpopulation.Thecataractsaresecondarytoaccumulationofgalactitolinthelenses.
Description
GALK1RecombinantHumanproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining412aminoacids(1-392a.a.)andhavingamolecularmassof44.4kDa.TheGALK1isfusedtoa20aminoacidHis-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMAALRQPQVAELLAEARRAFREEFGAEPELAVSAPGRVNLIGEHTDYNQGLVLPMALELMTVLVGSPRKDGLVSLLTTSEGADEPQRLQFPLPTAQRSLEPGTPRWANYVKGVIQYYPAAPLPGFSAVVVSSVPLGGGLSSSASLEVATYTFLQQLCPDSGTIAARAQVCQQAEHSFAGMPCGIMDQFISLMGQKGHALLIDCRSLETSLVPLSDPKLAVLITNSNVRHSLASSEYPVRRRQCEEVARALGKESLREVQLEELEAARDLVSKEGFRRARHVVGEIRRTAQAAAALRRGDYRAFGRLMVESHRSLRDDYEVSCPELDQLVEAALAVPGVYGSRMTGGGFGGCTVTLLEASAAPHAMRHIQEHYGGTATFYLSQAADGAKVLCL.