Cataloguenumber
PKA-093
Synonyms
cAMP-dependentproteinkinasealpha-catalyticsubunit,EC2.7.11.11,PKAC-alpha,PKACA,PRKACA, MGC48865,MGC102831.
Introduction
cAMP-dependentproteinkinasecatalyticsubunitalphaisoformCalpha1(PRKACA)belongstotheSer/Thrproteinkinasefamily.PRKACAisresponsIBLeforphosphorylatingotherproteinsandsubstrates,changingtheiractivity.ThePRKACAproteinisamemberoftheAGCkinasefamily,andcontributestotheregulationofcellularprocesseswhichincludeglucosemetabolism,celldivision,andcontextualmemory.
Description
PRKACAHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining578aminoacids(1-351a.a.)andhavingamolecularmassof67kDa(Migratesat50-70kDaonSDS-PAGEunderreducingconditions).PRKACAisexpressedwitha227aminoacidGSTTagatN-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
PRKACAproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85%asdeterminedbySDS-PAGE.
Aminoacidsequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSHMGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF.
