Cataloguenumber
PRO-930
Synonyms
Proteasomeassemblychaperone2,PAC-2,Hepatocellularcarcinoma-susceptibilityprotein3,Tumornecrosisfactorsuperfamilymember5-inducedprotein1,PSMG2,HCCA3,PAC2,TNFSF5IP1,CLAST3,MDS003,HsT1707,MGC15092.
Introduction
Proteasomeassemblychaperone2(PSMG2)stimulatesassemblyofthe20SproteasomeaspartofaheterodimerwithPSMG1.ThePSMG1-PSMG2heterodimerbindstothePSMA5andPSMA7proteasomesubunitsandpromotescompilationoftheproteasomealphasubunitsintotheheteroheptamericalpharingandpreventsalpharingdimerization.PSMG2iswidelyexpressedwithhighestlevelsinthelung,brainandcolon.Itismoderatelyexpressedinthemuscle,stomach,spleenandheart,andweaklyexpressedinthesmallintestine,pancreasandliver.Itisalsohighlyexpressedinhepatocellularcarcinomaswithlowlevelsinsurroundinglivertissue.
Description
PSMG2HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining287aminoacids(1-264a.a.)andhavingamolecularmassof31.8kDa.
PSMG2isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
PSMG2proteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0),40%glyceroland0.1MNaCl.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMFVPCGESAPDLAGFTLLMPAVSVGNVGQLAMDLIISTLNMSKIGYFYTDCLVPMVGNNPYATTEGNSTELSINAEVYSLPSRKLVALQLRSIFIKYKSKPFCEKLLSWVKSSGCARVIVLSSSHSYQRNDLQLRSTPFRYLLTPSMQKSVQNKIKSLNWEEMEKSRCIPEIDDSEFCIRIPGGGITKTLYDESCSKEIQMAVLLKFVSEGDNIPDALGLVEYLNEWLQILKPLSDDPTVSASRWKIPSSWRLLFGSGLPPALF.
