Cataloguenumber
PRO-931
Synonyms
Proteasomesubunitbetatype-10,Lowmolecularmassprotein10,MacropainsubunitMECl-1,MulticatalyticendopeptidasecomplexsubunitMECl-1,ProteasomeMECl-1,Proteasomesubunitbeta-2i,PSMB10,LMP10,MECL1,beta2i,MGC1665,FLJ00366.
Introduction
PSMB10isamemberoftheproteasomeB-typefamily(T1Bfamily)whichisa20Scorebetasubunit.Theproteasomeisamulticatalyticproteinasecomplexwithanextremelyorderedring-shaped20Scorestructure.Thiscorestructureiscomprisedoffourringsof28non-identicalsubunits;tworingsarecomposedofsevenalphasubunitsandtworingsarecomposedofsevenbetasubunits.ProteasomesarecirculatedineukaryoticcellsatahighconcentrationandcleavepeptidesinanATP/ubiquitin-dependentprocessinanon-lysosomalpathway.Acrucialfunctionofamodifiedproteasome,theimmunoproteasome,istheprocessingofclassIMHCpeptides.PSMB10geneexpressionisinducedbyinterferon-gamma,anditreplacescatalyticsubunit2(proteasomebeta7subunit)intheimmunoproteasome.
Description
PSMB10HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining255aminoacids(40-273a.a.)andhavingamolecularmassof26.9kDa.
PSMB10isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
PSMB10proteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0),40%glyceroland0.1MNaCl.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAV
TAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE.