Cataloguenumber
ENZ-103
Synonyms
CalcineurinsubunitBtype2,CalcineurinB-likeprotein,CBLP,CalcineurinBII,CNBII,PPP3R1-like,Proteinphosphatase2Bregulatorysubunit2,Proteinphosphatase3regulatorysubunitBbetaisoform,PPP3R2,PPP3RL.
Introduction
PPP3R2isacalcium-dependent,calmodulinstimulatedserine/threonineproteinphosphatase.Ineukaryotes,thephosphorylationanddephosphorylationofproteinsonserineandthreonineresiduesisacrucialmeansofregulatingawiderangeofcellularfunctionsincludingdivision,homeostasisandapoptosis.Ordinarilytheproteinphosphatase(PP)holoenzymeisatrimericcomplexcomposedofaregulatorysubunit,avariablesubunitandacatalyticsubunit.
Description
PPP3R2HumanRecombinantfusedwitha20a.a.HistagatN-terminusproducedinE.Coliisasingle,non-glycosylated,polypeptidechain(a.a.1-173)containing193aminoacidsandhavingamolecularmassof22kDa.
ThePPP3R2ispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
ThePPP3R2solution(0.5mg/ml)contains20mMTris-HClbuffer(pH8.0),10%glyceroland1mMDTT.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSTMGNEASYPAEMCSHFDNDEIKRLGRRFKKLDLDKSGSLSVEEFMSLPELRHNPLVRRVIDVFDTDGDGEVDFKEFILGTSQFSVKGDEEQKLRFAFSIYDMDKDGYISNGELFQVLKMMVGNNLTDWQLQQLVDKTIIILDKDGDGKISFEEFSAVVRDLEIHKKLVLIV.