Cataloguenumber
ENZ-1041
Synonyms
PHP14,CGI-202,HSPC141,PhosphohistidinePhosphatase1,PhosphohistidinePhosphatase14kDa,Proteinjanus-Ahomolog,Sex-regulatedproteinJanus-a.
Introduction
PHPT1isamemberoftheJanusproteinfamilyandis125aminoacidlong.PHPT1appearsasamonomerinthecytoplasmandisanEDTA-insensitivephosphohistidinephosphatase.OverexpressionofPHPT1resoltsinspecificphosphohistidinephosphataseactivitytowardsphosphopeptideI,withnoactivitydetectedtowardsphosphotyrosine,phosphothreonineandphosphoserinepeptides.
Description
PHPT1producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining145aminoacids(1-125a.a.)andhavingamolecularmassof15.9kDa.
PHPT1isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
ThePHPT1proteinsolution(0.5mg/ml)isformulatedin20mMTris-HClbuffer(pH8.0),0.2MNaCl,2mMDTTand10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
BIOLOGicalActivity
Specificactivityis>120units/mg,andisdefinedastheamountofenzymethathydrolyze1nmolfp-nitrophenylphosphateperminuteatpH7.5at37°C.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.