Cataloguenumber
ENZ-1044
Synonyms
Glycerol-3-phosphatephosphatase,G3PP,Aspartate-basedubiquitousMg(2+)-dependentphosphatase,AUM,Phosphoglycolatephosphatase,PGP.
Introduction
Phosphoglycolatephosphatase(PGP)isdiscoveredinalltissuesincludingredcells,lymphocytesandculturedfibroblasts(atproteinlevel).PGPismostactiveinskeletalmuscleandcardiacmuscle.ThecatalyticactivityofPGPis2-phosphoglycolate+H2O=glycolate+phosphate.DiseasesassociatedwithPGPincludetardivedyskinesiaandpolycystickidneydisease.
Description
PGPHumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining345aminoacids(1-321a.a)andhavingamolecularmassof36.5kDa.
PGPisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
PGPproteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),0.15MNaCl,10%glyceroland1mMDTT..
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
BIOLOGicalActivity
Specificactivityis>3,000units/mg,andisdefinedastheamountofenzymethathydrolyze1.0nmoleofp-nitrophenylphosphate(pNPP)perminuteatpH7.5at37C.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMAAAEAGGDDARCVRLSAERAQALLADVDTLLFDCDGVLWRGETAVPGAPEALRALRARGKRLGFITNNSSKTRAAYAEKLRRLGFGGPAGPGASLEVFGTAYCTALYLRQRLAGAPAPKAYVLGSPALAAELEAVGVASVGVGPEPLQGEGPGDWLHAPLEPDVRAVVVGFDPHFSYMKLTKALRYLQQPGCLLVGTNMDNRLPLENGRFIAGTGCLVRAVEMAAQRQADIIGKPSRFIFDCVSQEYGINPERTVMVGDRLDTDILLGATCGLKTILTLTGVSTLGDVKNNQESDCVSKKKMVPDFYVDSIADLLPALQG.

