Cataloguenumber
ENZ-1043
Synonyms
Dualspecificityproteinphosphatase23,Lowmolecularmassdualspecificityphosphatase3,LDP-3,VH1-likephosphataseZ,DUSP23,LDP3,VHZ,VH1-LikeMemberZ,EC3.1.3.16,EC3.1.3.48,DUSP25,MOSP,LDP3,DualSpecificityPhosphatase23,VH1-LikePhosphataseZ,LDP-3,VHZ,Low-Molecular-MassDual-SpecificityPhosphatase3,LowMolecularMassDualSpecificityPhosphatase3,DualSpecificityProtein,Phosphatase23,TesticularTissueProteinLi59.
Introduction
DUSP23isamemberoftheprotein-tyrosinephosphatasefamily.DUSP23isaproteinphosphatasewhichfacilitatesdephosphorylationofphosphorylatedproteinsonTyrandSer/Thrresidues.Invitro,DUSP23dephosphorylatep44-ERK1(MAPK3)butnotp54SAPK-beta(MAPK10).Inaddition,DUSP23enhancesactivationofJNKandp38(MAPK14).
Description
DUSP23HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining170aminoacids(1-150a.a)andhavingamolecularmassof18.8kDa.
DUSP23isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
DUSP23proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),2mMDTT,10%glyceroland100mMNaCl.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>200units/mg,andisdefinedastheamountofenzymethathydrolyzes1.0nmoleofp-nitrophenylphosphate(pNPP)perminuteatpH7.5at37°C.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGVQPPNFSWVLPGRLAGLALPRLPAHYQFLLDLGVRHLVSLTERGPPHSDSCPGLTLHRLRIPDFCPPAPDQIDRFVQIVDEANARGEAVGVHCALGFGRTGTMLACYLVKERGLAAGDAIAEIRRLRPGSIETYEQEKAVFQFYQRTK.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

