Cataloguenumber
ENZ-1053
Synonyms
MembraneMetalloendopeptidase,CommonAcuteLymphocyticLeukemiaAntigen,NeutralEndopeptidase24.11,SkinFibroblastElastase,NeutralEndopeptidase,Atriopeptidase,Enkephalinase,EC3.4.24.11,Neprilysin,CALLA,NEP,SFE,MembraneMetallo-Endopeptidase(NeutralEndopeptidase,Enkephalinase,CALLA,CD10),MembraneMetallo-EndopeptidaseVariant1,MembraneMetallo-EndopeptidaseVariant2,Neprilysin-390,Neprilysin-411,CD10Antigen,EC3.4.24,CMT2T,SCA43,CD10,EPN,MME.
Introduction
MembraneMetalloendopeptidase,alsoknownasMMEisazincmetallopeptidasewhichisexpressedatthecellsurfaceofvariouscells.MMEdegradestheamyloidbetapeptidewhoseabnormalmisfoldingaswellasaggregationinneuraltissuehasbeenimplicatedasthecauseforAlzheimer"sdisease.MMEisexpressedinanextendedrangeoftissuesandisespeciallyplentifulinthekidney.MMEisalsoacommonacutelymphocyticleukemiaantigenwhichisasignificantcellsurfaceMarkerinthediagnosisofhumanacutelymphocyticleukemia(ALL).MMEisusedinhematologicaldiagnosisbecauseitisexpressedbyearlyB,pro-Bandpre-Blymphocytes,andalsobylymphnodegerminalcenters.
Description
MMEHumanRecombinantproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining708aminoacids(52-750a.a.)andhavingamolecularmassof80.9kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).
MMEisexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Formulation
MMEproteinsolution(1mg/ml)20mMTris-HClbuffer(pH8.0)containing100mMNaCl,0.1mMPMSFand10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPYDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATW
RRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVWHHHHHH
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
