Cataloguenumber
ENZ-1035
Synonyms
Luciferase-likemonooxygenase,LUC,EC1.13.12.7.
Introduction
Luciferaseisageneraltermfortheclassofoxidativeenzymesusedinbioluminescenceandisdistinctfromaphotoprotein.LuciferasecatalyzesabioluminescentreactionwhichinvolvesthesubstrateluciferinaswellasMg2+andATP,producesgreenlightwithawavelengthof562nm.Luciferasefromfireflyisbroadlyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.
Description
LuciferaseproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining335aminoacids(1-311a.a)andhavingamolecularmassof38.5kDa.
Luciferaseisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheLuciferasesolution(0.5mg/ml)contains20mMTris-HCl(pH8.0),1mMDTTand10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ.
BIOLOGicalActivity
Specificactivityis>1x109lightunits/mg.OneluciferaseenzymeunitswillproduceoneRelativeLightUnit(RLU)atpH7.5at25°C.

