Cataloguenumber
ENZ-126
Synonyms
Diphosphoinositolpolyphosphatephosphohydrolase3-alpha,DIPP-3-alpha,DIPP3-alpha,hDIPP3alpha,Diadenosine5",5"""-P1,P6-hexaphosphatehydrolase3-alpha,Nucleosidediphosphate-linkedmoietyXmotif10,Nudixmotif10,hAps2,NUDT10,APS2,DIPP3A.
Introduction
NUDT10belongstotheNudixhydrolasefamilyofpyrophosphatases.NudixhydrolasescontainacharacteristicNudixdomainandareresponsIBLeforcatalyzingthehydrolysisofnucleosidediphosphatederivatives.NUDT10functionsasamanganese-dependentpolyphosphatephosphohydrolasewithanoptimumpHof8.5.NUDT10proteinspecificallymetabolizesdiadendosine-polyphosphatesand,toalesserextent,diphosphoinositolpolyphosphates.
Description
NUDT10producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining172aminoacids(1-164a.a.)andhavingamolecularmassof19.5kDa.
NUDT10isfusedtoan8aminoacidHis-tagatC-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
NUDT10proteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glycerol,0.1MNaCland1mMDTT.
StABIlity
NUDT10HumanRecombinantalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
Purity
Aminoacidsequence
MKCKPNQTRTYDPEGFKKRAACLCFRSEREDEVLLVSSSRYPDRWIVPGGGMEPEEEPGGAAVREVYEEAGVKGKLGRLLGVFEQNQDPKHRTYVYVLTVTELLEDWEDSVSIGRKREWFKVEDAIKVLQCHKPVHAEYLEKLKLGGSPTNGNSMAPSSPDSDPLEHHHHHH.