Cataloguenumber
ENZ-1056
Synonyms
3-Hydroxymethyl-3-Methylglutaryl-CoALyase,3-Hydroxymethyl-3-Methylglutaryl-CoenzymeALyase,3-Hydroxy-3-Methylglutarate-CoALyase,Hydroxymethylglutaricaciduria,HMG-CoALyase,EC4.1.3.4,HL,Mitochondrial3-Hydroxy-3-Methylglutaryl-CoALyase,Hydroxymethylglutaryl-CoALyase,Mitochondrial, 3-Hydroxy-3-Methylglutaryl-CoALyase,Hydroxymethylglutaryl-CoAlyase,mitochondrial,HMG-CoAlyase,3-hydroxy-3-methylglutarate-CoAlyase.
Introduction
Description
HMGCLHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining305aminoacids(28-325a.a.)andhavingamolecularmassof32.5kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).HMGCLisexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
HMGCLproteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4),20%glyceroland1mMDTT.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Aminoacidsequence
MTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKLHHHHHH.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.

