Cataloguenumber
ENZ-1051
Synonyms
ERp57,ERp60,ERp61,GRP57,GRP58,HsT17083,P58,PI-PLC,ER60,Proteindisulfide-isomeraseA3,DisulfideisomeraseER-60,Endoplasmicreticulumresidentprotein60,ERprotein60,58kDamicrosomalprotein,Endoplasmicreticulumresidentprotein57,ERprotein57,58kDaglucose-regulatedprotein,PDIA3.
Introduction
PDIA3isanenzymethatbelongstotheendoplasmicreticulumandinteractswithlectinchaperonescalreticulinandcalnexintomodulatefoldingofnewlysynthesizedglycoproteins.PDIA3hasproteindisulfideisomeraseactivity.ComplexesoflectinsandPDIA3mediateproteinfoldingbypromotingformationofdisulfidebondsintheirglycoproteinsubstrates.PDIA3isexpressedinthelumbarspinalcordfromratssubmittedtoperipherallesionduringneonatalperiod.PDIA3interactswiththiazide-sensitivesodium-chloridecotransporterinthekidneyandisinducedbyglucosedeprivation.PDIA3ispartofthemajorhistocompatibilitycomplex(MHC)classIpeptide-loADIngcomplex(TAP1),whichisimportantforformationofthefinalantigenconformationandexportfromtheendoplasmicreticulumtothecellsurface.
Description
PDIA3MouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining505aminoacids(25-505a.a)andhavingamolecularmassof56.8kDa.
PDIA3isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
PDIA3proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,0.1MNaCland10%glycerol.
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>15A650/cm/min/mg,obtainedbymeasuringtheincreaseofinsulinprecipitationinabsorbanceat650nmresultingfromthereductionofinsulin.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMSDVLELTDENFESRVSDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASVVGFFRDLFSDGHSEFLKAASNLRDNYRFAHTNIESLVKEYDDNGEGITIFRPLHLANKFEDKTVAYTEKKMTSGKIKKFIQDSIFGLCPHMTEDNKDLIQGKDLLTAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTTGEVPVVAIRTAKGEKFVMQEEFSRDGKALEQFLQEYFDGNLKRYLKSEPIPESNEGPVKVVVAENFDDIVNEEDKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVKGFPTIYFSPANKKLTPKKYEGGRELNDFISYLQREATNPPIIQEEKPKKKKKAQEDL.
