Cataloguenumber
ENZ-1046
Synonyms
Enoyl-CoAHydratase,ShortChain1,EnoylCoenzymeAHydratase,ShortChain,1,Mitochondrial,Enoyl-CoAHydratase,ShortChain,1,Mitochondrial,ShortChainEnoyl-CoAHydratase,EC4.2.1.17,SCEH,Enoyl-CoAHydratase,Mitochondrial,Short-ChainEnoyl-CoAHydratase,Enoyl-CoAHydratase1,EC4.2.1,ECHS1D,ECHS1 .
Introduction
Description
ECHS1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining284aminoacids(28-290a.a)andhavingamolecularmassof30.6kDa.ECHS1isfusedtoa21aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
ECHS1proteinsolution(1mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,20%Glyceroland100mMNaCl.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
BIOLOGicalActivity
Specificactivityis>150units/mg,andisdefinedastheamountofenzymethathydrolyzes1.0umoleofcrotonoyl-CoAtohydroxybutyryl-CoAperminuteperminuteatpH7.5at25°C.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

