Cataloguenumber
ENZ-1026
Synonyms
Histonedeacetylase8,HD8,Hdac8.
Introduction
Histonedeacetylase8(HDAC8)isamemberoftheclass1ofthehistonedeacetylase/acuc/aphafamily.HDAC8isBIOLOGicallyinvolvedinskullmorphogenesisandmetaboliccontroloftheERR-alpha/PGC1-alphatranscriptionalcomplex.Histonesplayakeyroleintranscriptionalregulation,cellcycleprogression,anddevelopmentalevents.Histoneacetylation/deacetylationmodifieschromosomestructureandaffectstranscriptionfactoraccesstoDNA.
Description
HDAC8MouseRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining383aminoacids(1-377a.a.)andhavingamolecularmassof42.5kDa(Migratesat40-57kDaonSDS-PAGEunderreducingconditions).
HDAC8isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
HDAC8proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MEMPEEPANSGHSLPPVYIYSPEYVSICDSLVKVPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDEDHPDSIEYGLGYDCPATEGIFDYAAAIGGGTITAAQCLIDGKCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFDRILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDMSDVGLGKGRYYSVNVPIQDGIQDEKYYHICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYVLQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVVHHHHHH.