Cataloguenumber
ENZ-1031
Synonyms
GZMK,TRYP2,Granzyme-K,GranzymeK,Fragmentin-3,Granzyme-3,NK-tryptase-2,NK-Tryp-2.
Introduction
GZMKisapartofagroupofrelatedserineproteasesfromthecytoplasmicgranulesofcytotoxiclymphocytes.CytolyticTlymphocytesandnaturalkillercellshavetheoutstandingABIlitytobind,recognizeandlysespecifictargetcells.Theydefendtheirhostbylysingcellsbearingontheirsurface"nonself"antigens,usuallypeptidesorproteinsresultingfrominfectionbyintracellularpathogens.
Description
GZMKHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining247aminoacids(27-264)andhavingamolecularmassof26.9kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).GZMKisfusedtoa6aminoacidIgGHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
GZMKproteinsolution(0.25mg/ml)containingPhosphateBufferedSaline(pH7.4),20%glyceroland1mMDTT.
Stability
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
ADPIIGGKEVSPHSRPFMASIQYGGHHVCGGVLIDPQWVLTAAHCQYRFTKGQSPTVVLGAHSLSKNEASKQTLEIKKFIPFSRVTSDPQSNDIMLVKLQTAAKLNKHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVLSRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSGGPLICKGVFHAIVSGGHECGVATKPGIYTLLTKKYQTWIKSNLVPPHTNHHHHHH.