Cataloguenumber
ENZ-1048
Synonyms
HMMH,HOGG1,MUTM,OGH1,APlyase,OGG1,8-OxoguanineDNAGlycosylase,OGG1.
Introduction
OGG1isaDNAglycosylaseenzymewhichtakespartinbaseexcisionrepair.OGG1proteinisthemainenzymeaccountablefortheexcisionof7,8-dihydro-8-oxoguanine(8-oxoG),amutagenicbasebyproductwhicharisesasaresultofexposuretoreactiveoxygenspecies(ROS).OGG1showsbetalyaseactivitythatnicksDNA3tothelesion.
Description
OGG1MouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining368aminoacids(1-345a.a)andhavingamolecularmassof41.3kDa.OGG1isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
OGG1proteinsolution(0.25mg/ml)containingPhosphateBufferedSaline(pH7.4)and30%glycerol.
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMLFRSWLPSSMRHRTLSSSPALWASIPCPRSELRLDLVLASGQSFRWKEQSPAHWSGVLADQVWTLTQTEDQLYCTVYRGDDSQVSRPTLEELETLHKYFQLDVSLAQLYSHWASVDSHFQRVAQKFQGVRLLRQDPTECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPNLHALAGPEAETHLRKLGLGYRARYVRASAKAILEEQGGPAWLQQLRVAPYEEAHKALCTLPGVGAKVADCICLMALDKPQAVPVDVHVWQIAHRDYGWHPKTSQAKGPSPLANKELGNFFRNLWGPYAGWAQAVLFSADLRQPSLSREPPAKRKKGSKRPEG.

