Cataloguenumber
PKA-101
Synonyms
CD333Antigen,HSFGFR3EX,EC2.7.10,CD333,CEK2,ACH,FibroblastGrowthFactorReceptor3,EC2.7.10.1,FGFR-3,JTK4,FibroblastGrowthFactorReceptor3Variant4,Achondroplasia,ThanatophoricDwarfism,Hydroxyaryl-ProteinKinase,TyrosineKinaseJTK4.
Introduction
FibroblastGrowthFactors(FGFs)compriseafamilyofatleasteighteenstructurallyrealtedproteinsthatareinvolvedinamultitudeofphysiologicalandpathologicalcellularprocesses,includingcellgrowth,differentation,angiogenesis,woundhealingandtumorgenesis.TheBIOLOGicalactivitiesoftheFGFsaremediatedbyafamilyiftypeItransmembranetyrosinekinaseswhichundergodimerizationandautophosphorylationafterligandbinding.FourdistinctgenesencodingcloselyrelatedFGFreceptors,FGFR-1to-4areknown.MultipleformsofFGFR-1to-3aregeneratedbyalternativesplicingofthemRNAs.AfrequentsplicingeventinvolvingFGFR-1and-2resultsinreceptorscontainingallthreeIgdomains,referredtoasthealphaisoform,oronlyIgIIandIgIII,referredtoasthe?isoform.OnlythealphaisoformhasbeenidentifiedforFGFR-3andFGFR-4.AdditionalsplicingeventsforFGFR-1to-3,involvingtheC-terminalhalfoftheIgIIIdomainencodedbytwomutuallyexclusivealternativeexons,generateFGFreceptorswithalternativeIgIIIdomains(IIIbandIIIc).AIIIaisoformwhichisasecretedFGFbindingproteincontainingonlytheN-terminalhalfoftheIgIIIdomainplussomeintronsequenceshasalsobeenreportedforFGFR-1.MutationsinFGFR-1to-3havebeenfoundinpatientswithbirthdefectsinvolvingcraniosynostosis.
Description
FGFR3HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining592aminoacids(23-375a.a.)andhavingamolecularmassof65.1kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).
FGFR3isexpressedwitha239aminoacidhIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
FGFR3proteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ESLGTEQRVVGRAAEVPGPEPGQQEQLVFGSGDAVELSCPPPGGGPMGPTVWVKDGTGLVPSERVLVGPQRLQVLNASHEDSGAYSCRQRLTQRVLCHFSVRVTDAPSSGDDEDGEDEAEDTGVDTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAGNPTPSISWLKNGREFRGEHRIGGIKLRHQQWSLVMESVVPSDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPILQAGLPANQTAVLGSDVEFHCKVYSDAQPHIQWLKHVEVNGSKVGPDGTPYVTVLKTAGANTTDKELEVLSLHNVTFEDAGEYTCLAGNSIGFSHHSAWLVVLPAEEELVEADEAGSVYAGLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
