Cataloguenumber
PKA-098
Synonyms
EC2.7.10,FGFR-2,BFR-1,CD332,BBDS,CEK3,ECT1,TK14,TK25,CFD1,KSAM,JWS,FibroblastGrowthFactorReceptor2,KeratinocyteGrowthFactorReceptor,Bacteria-ExpressedKinase,EC2.7.10.1,K-SAM,KGFR,BEK,ProteinTyrosineKinase,ReceptorLike14,BEKFibroblastGrowthFactorReceptor,CraniofacialDysostosis1,Jackson-WeissSyndrome,PfeifferSyndrome,CrouzonSyndrome,CD332Antigen.
Introduction
FibroblastGrowthFactors(FGFs)compriseafamilyofatleasteighteenstructurallyrealtedproteinsthatareinvolvedinamultitudeofphysiologicalandpathologicalcellularprocesses,includingcellgrowth,differentation,angiogenesis,woundhealingandtumorgenesis.TheBIOLOGicalactivitiesoftheFGFsaremediatedbyafamilyiftypeItransmembranetyrosinekinaseswhichundergodimerizationandautophosphorylationafterligandbinding.FourdistinctgenesencodingcloselyrelatedFGFreceptors,FGFR-1to-4areknown.MultipleformsofFGFR-1to-3aregeneratedbyalternativesplicingofthemRNAs.AfrequentsplicingeventinvolvingFGFR-1and-2resultsinreceptorscontainingallthreeIgdomains,referredtoasthealphaisoform,oronlyIgIIandIgIII,referredtoasthe?isoform.OnlythealphaisoformhasbeenidentifiedforFGFR-3andFGFR-4.AdditionalsplicingeventsforFGFR-1to-3,involvingtheC-terminalhalfoftheIgIIIdomainencodedbytwomutuallyexclusivealternativeexons,generateFGFreceptorswithalternativeIgIIIdomains(IIIbandIIIc).AIIIaisoformwhichisasecretedFGFbindingproteincontainingonlytheN-terminalhalfoftheIgIIIdomainplussomeintronsequenceshasalsobeenreportedforFGFR-1.MutationsinFGFR-1to-3havebeenfoundinpatientswithbirthdefectsinvolvingcraniosynostosis.
Description
FGFR2HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining596aminoacids(22-378a.a.)andhavingamolecularmassof66.6kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).
FGFR2isexpressedwitha239aminoacidshIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
FGFR2proteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
RPSFSLVEDTTLEPEEPPTKYQISQPEVYVAAPGESLEVRCLLKDAAVISWTKDGVHLGPNNRTVLIGEYLQIKGATPRDSGLYACTASRTVDSETWYFMVNVTDAISSGDDEDDTDGAEDFVSENSNNKRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCVVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTEADAGEYICKVSNYIGQANQSAWLTVLPKQQAPGREKEITASPDYLELEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
