Cataloguenumber
PKA-100
Synonyms
FGFR-1,bFGF-R,C-FGR,CD331,fms-relatedtyrosinekinase2,Pfeiffersyndrome,CEK,FLG,FLT2,KAL2,BFGFR,FGFBR,HBGFR,FGFR1/FGFR1OP2FUSIONGENE,FGFR1/ZNF198FUSIONGENE,FLGFGFR1/BCRFUSIONGENE,FLGprotein,FMS-LIKEGENE,N-samtyrosinekinase,basicfibroblastgrowthfactorreceptor1.
Introduction
FibroblastGrowthFactors(FGFs)compriseafamilyofatleasteighteenstructurallyrealtedproteinsthatareinvolvedinamultitudeofphysiologicalandpathologicalcellularprocesses,includingcellgrowth,differentation,angiogenesis,woundhealingandtumorgenesis.TheBIOLOGicalactivitiesoftheFGFsaremediatedbyafamilyiftypeItransmembranetyrosinekinaseswhichundergodimerizationandautophosphorylationafterligandbinding.FourdistinctgenesencodingcloselyrelatedFGFreceptors,FGFR-1to-4areknown.MultipleformsofFGFR-1to-3aregeneratedbyalternativesplicingofthemRNAs.AfrequentsplicingeventinvolvingFGFR-1and-2resultsinreceptorscontainingallthreeIgdomains,referredtoasthealphaisoform,oronlyIgIIandIgIII,referredtoasthe?isoform.OnlythealphaisoformhasbeenidentifiedforFGFR-3andFGFR-4.AdditionalsplicingeventsforFGFR-1to-3,involvingtheC-terminalhalfoftheIgIIIdomainencodedbytwomutuallyexclusivealternativeexons,generateFGFreceptorswithalternativeIgIIIdomains(IIIbandIIIc).AIIIaisoformwhichisasecretedFGFbindingproteincontainingonlytheN-terminalhalfoftheIgIIIdomainplussomeintronsequenceshasalsobeenreportedforFGFR-1.MutationsinFGFR-1to-3havebeenfoundinpatientswithbirthdefectsinvolvingcraniosynostosis.
Description
FGFRHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining363aminoacids(22-376)andhavingamolecularmassof40.4kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).FGFRisfusedtoa8aminoacidHis-tagatC-terminus&purifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
TheFGFRsolution(0.5mg/1ml)containsphosphatebufferedSaline(pH7.4),and10%glycerol.
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
RPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLELEHHHHHH.
