Cataloguenumber
ENZ-1027
Synonyms
15-hydroxyprostaglandindehydrogenase[NAD(+)](EC:1.1.1.141),15-PGDH,Hpgd,Pgdh1,Prostaglandindehydrogenase1.
Introduction
HPGDistheessentialenzymeofprostaglandindegradation.15-PGDHproteinstronglydecreasestheBIOLOGicactivityofthesemoleculesbycatalyzingtheoxidationofthe15-hydroxylgroupofprostaglandinstoaketogroup.GDH1isinvolvedinnumerousphysiologicandcellularprocesses,forinstanceinflammation.
Description
HPGDMouseRecombinantproducedinE.coliisasinglepolypeptidechaincontaining292aminoacids(1-269)andhavingamolecularmassof31.6kDa.
HPGDisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
Formulation
TheHPGDsolution(1mg/ml)contains20mMTris-HClbuffer(pH8.0)and10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMHVNGKVALVTGAAQGIGKAFAEALLLHGAKVALVDWNLEAGVKCKAALDEQFEPQKTLFVQCDVADQKQLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEQTLQINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLAGLMPVAQQPVYCASKHGIIGFTRSAAMAANLMKSGVRLNVICPGFVDTPILESIEKEENMGQYIEYKDQIKAMMKFYGVLHPSTIANGLINLIEDDALNGAIMKITASKGIHFQDYDISPLLVKAPLTS.

