Cataloguenumber
ENZ-1058
Synonyms
Guaninedeaminase,Guanase,Guanineaminase,Guanineaminohydrolase,GAH.
Introduction
Description
GDAMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining477aminoacids(1-454a.a)andhavingamolecularmassof53.4kDa.
GDAisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
GDAproteinsolution(0.5mg/ml)containing20mMTris-HCl(pH8.0),0.15MNaCl,1mMDTTand10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>4,000pmol/min/ug,andisdefinedas
theamountofenzymethatconvertguaninetoxanthineperminuteatpH8.0at37°C.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMCAARTPPLALVFRGTFVHSTWTCPMEVLRDHLLGVSDSGKIVFLEESSQQEKLAKEWCFKPCEIRELSHHEFFMPGLVDTHIHAPQYAFAGSNVDLPLLEWLNKYTFPTEQRFRSTDVAEEVYTRVVRRTLKNGTTTACYFGTIHTDSSLILAEITDKFGQRAFVGKVCMDLNDTVPEYKETTEESVKETERFVSEMLQKNYPRVKPIVTPRFTLSCTETLMSELGNIAKTHDLYIQSHISENREEIEAVKSLYPSYKNYTDVYDKNNLLTNKTVMAHGCYLSEEELNIFSERGASIAHCPNSNLSLSSGLLNVLEVLKHKVKIGLGTDVAGGYSYSMLDAIRRAVMVSNVLLINKVNEKNLTLKEVFRLATLGGSQALGLDSEIGNFEVGKEFDALLINPRASDSPIDLFYGDFVGDISEAVIQKFLYLGDDRNIEEVYVGGKQVVPFSSSV.

