Cataloguenumber
ENZ-1045
Synonyms
Peptidyl-prolylcis-transisomeraseNIMA-interacting1(EC:5.2.1.8),Peptidyl-prolylcis-transisomerasePin1,PPIasePin1,Pin1,PIN1.
Introduction
Description
PIN1MouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining188aminoacids(1-165a.a)andhavingamolecularmassof20.8kDa.PIN1isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
PIN1proteinsolution(1mg/ml)containingPhosphatebufferedsaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
BIOLOGicalActivity
Specificactivityis>1,200nmol/min/mg,andisdefinedastheamountofenzymethatcleaves1nmoleofsuc-AAFP-PNAperminuteat37°CinTris-HClpH8.0usingchymotrypsin.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGGSTVGGSSKNGQGEPAKVRCSHLLVKHSQSRRPSSWRQEKITRSKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGPFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.