Cataloguenumber
ENZ-1033
Synonyms
Beta-lactamase,Cephalosporinase,ampC,ampA,b4150,JW4111.
Introduction
Beta-lactamaseisatypeofenzyme(EC3.5.2.6)producedbysomebacteriathatisresponsIBLefortheirresistancetobeta-lactamantibioticslikepenicillins,cephalosporins,cephamycinsandcarbapenems.Theseantibioticshaveacommonelementintheirmolecularstructure:afour-atomringknownasabeta-lactam.Thelactamaseenzymebreaksthatringopen,deactivatingthemolecule"santibacterialproperties.
Description
LACTBproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining379aminoacids(20-377a.a)andhavingamolecularmassof41.8kDa.
LACTBisfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheLACTBsolution(1mg/ml)contains20mMTris-HClbuffer(pH8.0)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMAPQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ.
BIOLOGicalActivity
Specificactivityis>700units/mg,inwhichOneunitwillhydrolyze1.0umoleofNitrocefinperminuteatpH7.0at37°C.

