Cataloguenumber
ENZ-1054
Synonyms
EC3.3.1.1,SAHH,AdoHcyase,S-adenosyl-L-homocysteinehydrolase,Adenosylhomocysteinase.
Introduction
AHCYisanenzymethatcatalyzesthereversIBLehydrolysisofS-adenosylhomocysteine(AdoHcy)toadenosine(Ado)andL-homocysteine(Hcy).AHCYcontrolstheintracellularS-adenosylhomocysteine(SAH)concentrationthatiscrucialfortransmethylationreactions.AHCYdeficiencycauseshypermethioninemia.
Description
AHCYMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining456aminoacids(1-432a.a)andhavingamolecularmassof50.2kDa.
AHCYisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
AHCYproteinsolution(0.25mg/ml)containing20mMTris-Hclbuffer(pH8.0)containing40%glycerol0.2MNaCl,1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorage,itisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMREMYSASKPLKGARIAGCLHMTVETAVLIETLVALGAEVRWSSCNIFSTQDHAAAAIAKAGIPVFAWKGETDEEYLWCIEQTLHFKDGPLNMILDDGGDLTNLIHTKYPQLLSGIRGISEETTTGVHNLYKMMSNGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACKEGNIFVTTTGCVDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYWLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMPINGPFKPDHYRY.

