Cataloguenumber
PKA-095
Synonyms
YWHAE,MDS,MDCR,KCIP-1,14-3-3E,14-3-3Epsilon,FLJ45465,Tyr-3/Trp-5MonooxygenaseActivationProteinEpsilon,HEL2,KCIP-1,MDCR,MDS.
Introduction
The14-3-3familyofproteinsplaysakeyregulatoryroleinsignaltransduction,checkpointcontrol,apoptoticandnutrient-sensingpathways.14-3-3proteinsarehighlyconservedandubiquitouslyexpressed.Thereareatleastsevenisoforms, β,γ,ε,σ,ζ,τandη thathavebeenidentifiedinmammals.The14-3-3epsilon,asubtypeofthe14-3-3familyofproteins,wasthoughttobebrainandneuron-specific.IthasbeenshowntointeractwithCDC25phosphatases,RAF1andIRS1proteins,suggestingitsroleindiversebiochemicalactivitiesrelatedtosignaltransduction,suchascelldivisionandregulationofinsulinsensitivity.Ithasalsobeenimplicatedinthepathogenesisofsmallcelllungcancer.
Description
YWHAEHumanRecombinantproducedinE.coliisasinglepolypeptidechaincontaining275aminoacids(1-255)andhavingamolecularmassof31.3kDa.YWHAEisfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheYWHAEsolution(1mg/1ml)containsphosphatebufferedSaline(pH7.4),1mMDTTand10%glycerol.
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ.

