Cataloguenumber
PKA-096
Synonyms
14-3-3proteinbeta/alpha,ProteinkinaseCinhibitorprotein1,Protein1054, YWHAB,HS1,GW128,KCIP-1,Tyr-3/Trp-5MonooxygenaseActivationProteinBeta,14-3-3Beta.
Introduction
YWHABbelongstothe14-3-3familyofproteinswhichareinchargeforsignaltransductionbybindingtophosphoserine-containingproteins.YWHABisfoundinbothplantsandmammals.YWHABproteininteractswithRAF1andCDC25phosphatases,thuslinkingmitogenicsignalingandthecellcyclemachinery.YWHABisanadapterproteininvolvedintheregulationofalargespectrumofbothgeneralandspecializedsignalingpathway.YWHABbindstoalargenumberofproteinsbyrecognitionofaphosphoserineorphosphothreoninemotif.Bindinggenerallyresultsinthemodulationoftheactivityofthebindingpartner.
Description
YWHABHumanRecombinantproducedinE.coliisasinglepolypeptidechaincontaining270aminoacids(1-246)andhavingamolecularmassof30.6kDa.YWHABisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheYWHABsolution(1mg/1ml)containsphosphatebufferedSaline(pH7.4)and10%glycerol.
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN.

