
Cataloguenumber
HOR-020
Synonyms
GL-R,GLR,GlucagonReceptor.
Introduction
GlucagonreceptorispartoftheglucagonreceptorfamilywhichalsocontainsGLP-1,GLP-2,secretin,GHRHandGIPreceptors.GCGRregulatesbloodglucoselevelsandismainlyexpressedinthepancreas,liverandkidneys.MutationsinGCGRareacauseofnon-insulin-dependentdiabetesmellitus.
Description
GCGRHumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechain(a.a29-142)containing125aminoacidsincludingan11a.aC-terminalHistag.Thetotalmolecularmassis19.9kDa(calculated).
Source
E.coli
PhysicalAppearance
Formulation
GCGRfiltered(0.4µm)andlyophilizedfromsolutioninacetonitrile/0,1%TFAand1%(w/v)trehalose.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.GCGRisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeafteroneweekat4°C.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MQVMDFLFEKWKLYGDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDGQWVRGPRGQPWRDASQCQMDGEEIEVQKEVAKMYSSFQLEHHHHHH
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.