Cataloguenumber
CYT-257
Synonyms
Introduction
Description
TheNT-3ispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Solubility
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.
Aminoacidsequence
BIOLOGicalActivity
Proteincontent
1.UVspectroscopyat280nmusingtheabsorbencyvalueof2.165astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).
2.AnalysisbyRP-HPLC,usingastandardsolutionofNT-3asaReferenceStandard.
Usage
References
1.Title:GenerationofDopamineNeuronswithImprovedCellSurvivalandPhenotypeMaintenanceUsingaDegradation-ResistantNurr1Mutant[1]^†[2]^‡.
Publication:Articlefirstpublishedonline:11JUN2009DOI:10.1002/stem.146Copyright©2009AlphaMedPress.
Link:http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full
Links:
1.http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full#fn1
2.http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full#fn3
2.Title:Mash1andNeurogenin2EnhanceSurvivalandDifferentiationofNeuralPrecursorCellsAfterTransplantationtoRatBrainsviaDistinctModesofAction.
Publication:Received22May2008;Accepted8August2008;Publishedonline9September2008.
MolecularTherapy(2008)1611,1873–1882doi:10.1038/mt.2008.189
Link:http://www.nature.com/mt/journal/v16/n11/full/mt2008189a.html
3.Title:TrkAReceptor“HotSpots”forBindingofNT-3asaHeterologousLigand
Publications: JournalofBiologicalChemistry282.23(2007):16754-16763.
Link:http://www.jbc.org/content/282/23/16754.full
4.Title:OrphannuclearreceptorNurr1inducesneurondifferentiationfromembryoniccorticalprecursorcellsviaanextrinsicparacrinemechanism
Publication: FEBSletters583.9(2009):1505-1510
Link http://www.sciencedirect.com/science/article/pii/S0014579309002828