Cataloguenumber
CYT-1012
Synonyms
GDNFfamilyreceptoralpha-1,GDNFreceptoralpha-1,GDNFR-alpha-1,GFR-alpha-1,RETligand1,TGF-beta-relatedneurotrophicfactorreceptor1,Gfra1,Gdnfra,Retl1,Trnr1.
Introduction
GDNFfamilyreceptoralpha-1(GFRA1)belongstotheGDNFreceptorfamily.GFRA1isaglycosyl-phosphatidylinositol(GPI)-linkedcellsurfacereceptorforbothGlialcellline-derivedgrowthfactor(GDNF),neurturin(NTN),andmediatesactivationoftheRETtyrosinekinasereceptor.TheGFRA1proteinisapotentsurvivalfactorforcentralandperipheralneurons,andisvitalforthedevelopmentofkidneysandtheentericnervoussystem.
Description
GFRA1RatRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining645aminoacids(25-430a.a.)andhavingamolecularmassof72.3kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).GFRA1isexpressedwitha239aminoacidhIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
GFRA1proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85%asdeterminedbySDS-PAGE.
Aminoacidsequence
DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISDVFQQVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEERERPNCLSLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYVDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDSDFGKDGLAGASSLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
