
Cataloguenumber
PRO-2472
Synonyms
CD81Molecule,CD81Antigen(TargetOfAntiproliferativeAntibody1),26KDaCellSurfaceProteinTAPA-1,Tetraspanin-28,CD81Antigen,Tspan-28,TSPAN28,TAPA1,TargetOfTheAntiproliferativeAntibody1,CVID6,S5.7,26kDacellsurfaceproteinTAPA-1,Targetoftheantiproliferativeantibody1,Tetraspanin-28,Tspan-28.
Introduction
CD81antigenisoform1,alsoknownasCD81,ispartofthetetraspaninfamily.CD81interactsdirectlywithimmunoglobulinsuperfamilymember8(IGSF8,CD316)andCD36.InBcells,CD81ispartofacomplexwithCD21,CD19andLeu13.FurThermore,thiscomplexdecreasesthethresholdforBcellactivationthroughBcellreceptorsbylinkingAgspecificrecognitionandCD21mediatorrecognition.WhileonTcellsCD81connectswithCD4andCD8andprovidesacostimulatorysignalwithCD3.
Description
CD81producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining331aminoacids(113-201a.a.)andhavingamolecularmassof37.0kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
CD81isexpressedwitha242aminoacidhIgG-His-tagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CD81proteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPFVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGKLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRV VSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.