Cataloguenumber
PRO-2437
Synonyms
T-cell-specificsurfaceglycoproteinCD28,TP44,CD_antigen:CD28,T-cell-specificsurfaceglycoproteinCD28isoform1.
Introduction
CD28co-stimulationisnecessaryforCD4positiveT-cellproliferation&survival,interleukin-2production,andT-helpertype-2development.Humanpost-thymicregulatoryTcellsrequireCD28co-stimulationtoexpandandmaintainpotentsuppressivefunctioninvivo.Apoptosisplaysakeyroleintheage-relateddeclineofCD28expressionandinimmunosenescence.
Description
CD28HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining376aminoacids(19-152a.a.)andhavingamolecularmassof42.4kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).CD28isexpressedwitha242aminoacidshIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
CD28proteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPNKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.