Cataloguenumber
PRO-2444
Synonyms
CD99antigen,T-cellsurfaceglycoproteinE2,E2antigen,ProteinMIC2,12E7,CD99,MIC2,MIC2X,MIC2Y.
Introduction
CD99,orMIC2geneproduct,orE2antigenisexpressedonthecellmembraneofsomelymphocytes,corticalthymocytes,andgranulosacellsoftheovary.Theantigenisalsoexpressedbymostpancreaticisletcells,Sertolicellsofthetestis,andsomeendothelialcells.MaturegranulocytesexpressverylittleornoCD99.MIC2isstronglyexpressedonEwing’ssarcomacellsandprimitiveperipheralneuroectodermaltumors.
Description
CD99HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining342aminoacids(23-122a.a.)andhavingamolecularmassof37.3kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).
CD99isexpressedwitha239aminoacidshIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
CD99proteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPDGGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNPPKPMPNPNPNHPSSSGSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEADLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.