Cataloguenumber
PRO-2386
Synonyms
5"-nucleotidase(EC:3.1.3.5),5"-NT,Ecto-5"-nucleotidase,CD73,Nt5e,Nt5,Nte.
Introduction
Description
NT5EMouseRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining531aminoacids(29-551a.a.)andhavingamolecularmassof59.1kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).
NT5Eisexpressedwithan6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
NT5Eproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKFSLEHHHHHH.
BIOLOGicalActivity
Specificactivityis>8,000pmol/min/ug,andisdefinedastheamountofenzymethathydrolyze1.0pmoleofAdenosine5-monophosphatetophosphateperminuteperminuteatpH7.5at25°C.