Cataloguenumber
PRO-2453
Synonyms
CD274molecule,CD274antigen,programmedcelldeath1ligand1,PD-L1,PDCD1L1,PDCD1LG1,PDCD1ligand1,B7homolog1,B7H1.
Introduction
CD274takespartinthecostimulatorysignal,vitalforT-cellproliferationandcreationofIL10andIFNG,inaPDCD1-independentandanIL2-dependentmanner.CD274collaborationwithPDCD1inhibitscytokineproductionandT-cellproliferation.
Description
CD274HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining462aminoacids(19-238a.a.)andhavingamolecularmassof52.5kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).CD274isexpressedwitha239aminoacidshIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
CD274proteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADLFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.