Cataloguenumber
CYT-692
Synonyms
SolubleReceptorActivatorofNFkBLigand,TNFSF11,TRANCE,TNF-relatedactivation-inducedcytokine,OPGL,ODF,Osteoclastdifferentiationfactor,Tumornecrosisfactorligandsuperfamilymember11,ReceptoractivatorofnuclearfactorkappaBligand,RANKL,Osteoprotegerinligand,CD254antigen,sRANKL,sOdf,hRANKL2.
Introduction
RANKLbindstotnfrsf11b/opgandtotnfrsf11a/rank.Osteoclastdifferentiationandactivationfactor.AugmentstheABIlityofdendriticcellstostimulatenaivet-cellproliferation.Maybeanimportantregulatorofinteractionsbetweent-cellsanddendriticcellsandmayplayaroleintheregulationofthet-cell-dependentimmuneresponse.sRANKLmayalsoplayanimportantroleinenhancedbone-resorptioninhumoralhypercalcemiaofmalignancy.
Description
sRANKLHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining199aminoacids(140-317a.a)andhavingamolecularmassof22.3kDa.
sRANKLisfusedtoa21aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
sRANKLproteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),20%glycerol,0.1MNaCland1mMDTT.
Stability
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMIRAEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID.