Cataloguenumber
CYT-1031
Synonyms
PIGF,PGF,PLGF-1,PlGF-2.
Introduction
PLGFisagrowthfactoractiveinangiogenesis,andendothelialcellgrowth,stimulatingtheirproliferationandmigration.Itbindstoreceptorvegfr-1/flt1.
Description
PLGF1HumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechain(a.a19-131)containing123aminoacidsincludinga10a.aN-terminalHistag.Thetotalmolecularmassis13.8kDa(calculated).
Source
PhysicalAppearance
Formulation
PLGF1filtered(0.4µm)andlyophilizedfrom0.5mg/mlinPBSand5%w/vtrehalose,pH7.4.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.PLGF1isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MKHHHHHHASLPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECR.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.