Cataloguenumber
CYT-1033
Synonyms
MacrophageMigrationInhibitoryFactor(Glycosylation-InhibitingFactor),PhenylpyruvateTautomerase,L-DopachromeTautomerase,L-DopachromeIsomerase,GLIF,MMIF,GIF,MacrophageMigrationInhibitoryFactor,Glycosylation-InhibitingFactor,EC5.3.3.12,EC5.3.2.1,Macrophagemigrationinhibitoryfactor.
Introduction
ThecytokineMacrophagemigrationinhibitoryfactor(MIF)hasbeenidentifiedtobesecretedbythepituitaryglandandthemonocyte/macrophageandtoplayanimportantroleinendotoxicshock.MIFhastheuniquepropertyofbeingreleasedfrommacrophagesandTcellsinresponsetophysiologicalconcentrationsofglucocorticoids.ThesecretionofMIFistightlyregulatedanddecreasesathigh,anti-inflammatorysteroidconcentration.
Description
T.VaginalisMIFRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechain(a.a1-115)containing121aminoacidsincludinga6a.aC-terminalHistag.Thetotalmolecularmassis13.4kDa(calculated).
Source
PhysicalAppearance
Formulation
T.VaginalisMIFfiltered(0.4µm)andlyophilizedfrom0.5mg/mlsolutionin20mMTrisbufferand50mMNaCl,pH7.5.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.T.VaginalisMIFisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MPALVIKTNAKFTEEEKSKATEELGNIVSKVLGKPISYVMVTLEDGVAVRFGGSDEKAAFMSLMSIGGLNRAVNKRASAALTKWFTDHGFQGDRIYIVFNPKSAEDWGFNGDTFAHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.