Prospec/KGF Human/CYT-219/10µg
商品编号:
CYT-219-10µg
品牌:
prospecbio
市场价:
¥2600.00
美元价:
1170.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Cataloguenumber
CYT-219
Synonyms
HBGF-7,FGF7,FGF-7,KGF.
Introduction
KGFisamemberofthefibroblastgrowthfactor(FGF)family.FGFfamilymemberspossessbroadmitogenicandcellsurvivalactivities,andareinvolvedinavarietyofBIOLOGicalprocesses,includingembryonicdevelopment,cellgrowth,morphogenesis,tissuerepair,tumorgrowthandinvasion.FGF7isapotentepithelialcell-specificgrowthfactor,whosemitogenicactivityispredominantlyexhibitedinkeratinocytesbutnotinfibroblastsandendothelialcells.Studiesofmouseandrathomologsofthisgeneimplicatedrolesinmorphogenesisofepithelium,reepithelializationofwounds,hairdevelopmentandearlylungorganogenesis.
Description
KeratinocyteGrowthFactor-1HumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining164aminoacidsandhavingamolecularmassof18995Dalton.
TheFGF-7ispurifiedbyproprietarychromatographictechniques.
TheFGF-7ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2?mfilteredsolutionin20mMPB,pH8.0,1MNaCl.
Solubility
ItisrecommendedtoreconstitutethelyophilizedKeratinocyteGrowthFactorinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedKeratinocyteGrowthFactor1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFGF7shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan96.0%asdeterminedby:
(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.
(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.
Aminoacidsequence
MCNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT.
BiologicalActivity
Thebiologicalactivitywasdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingKGFreceptorsyieldinganED50<10ng/ml,correspondingtoaSpecificActivityof1.0×105IU/mg.
Proteincontent
Proteinquantitationwascarriedoutbytwoindependentmethods:
1.UVspectroscopyat280nmusingtheabsorbencyvalueof0.9astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).
2.AnalysisbyRP-HPLC,usingacalibratedsolutionofKGFasaReferenceStandard.
1.UVspectroscopyat280nmusingtheabsorbencyvalueof0.9astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).
2.AnalysisbyRP-HPLC,usingacalibratedsolutionofKGFasaReferenceStandard.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
References
1.Title:HumanCordBlood-DerivedEndothelialProgenitorCellsandTheirConditionedMediaExhibitTherapeuticEquivalenceforDiabeticWoundHealing.
Publication:
CellTransplantation
ISSN:0963-6897
DOI:10.3727/096368910X516637
Volume19,Issue12,pages1635-1644
Copyright©2010CognizantComm.Corp
Link:http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000012/art00011
2.Title:TransformingGrowthFactor-Alpha:AMajorHumanSerumFactorthatPromotesHumanKeratinocyteMigration.
Publication:JournalofInvestigativeDermatology(2006)126,2096–2105.doi:10.1038/sj.jid.5700350;publishedonline11May2006.
Link:http://www.nature.com/jid/journal/v126/n9/full/5700350a.html
Publication:
CellTransplantation
ISSN:0963-6897
DOI:10.3727/096368910X516637
Volume19,Issue12,pages1635-1644
Copyright©2010CognizantComm.Corp
Link:http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000012/art00011
2.Title:TransformingGrowthFactor-Alpha:AMajorHumanSerumFactorthatPromotesHumanKeratinocyteMigration.
Publication:JournalofInvestigativeDermatology(2006)126,2096–2105.doi:10.1038/sj.jid.5700350;publishedonline11May2006.
Link:http://www.nature.com/jid/journal/v126/n9/full/5700350a.html
品牌介绍
ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作,1947年出生于以色列。1991-1997创建ReProGen 公司,从事细胞因子和趋化因子的大量生产及纯化;1997-2000创建ProSpec-TechnoGene,主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。
ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.
Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....
Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.
ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.
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